HLP experts are the authors of pioneering works on innovative research and transfer of reproductive and stem cell technology in Vietnam.

HLP was developed by experienced experts in the field of modern biotechnology, updating the world's developments from embryo technology, cryobiology, and cloning in the 80s and 90s of the twentieth century to today's stem cell and genetic technology.

Our R&D Backgroung

HLP experts and collaborators have chaired and participated in many national and international projects working on the application of technological innovations for improving biodiversity conservation, livestock development and for biomedicine.

We have led various research and development (R&D) and technology transfer initiatives at both national and international collaboration levels, forging close partnerships with research counterparts in France, the USA, Japan, Thailand, Switzerland, Czechoslovakia, Hungary, Italia, Belgium , Korea, Germany and southeast asian countires

> Leading the way in human-assisted reproductive technology, with the initiation of the first successful semen cryobanking and its application for infertility treatment in 1999.

> Pioneering research in somatic cloning and cell reprogramming, marked by the first successful application of interspecies somatic cell nuclear transfer in wild endangered animal species during the years 1998-2000.

Currently, the team is a gathering place for young experts with doctoral training at leading, prestigious research centers in France, America, Japan, etc. A team ready to carry out the HLP's destiny.

> Pioneering research in embryo biotechnology, achieving the first successful embryo transfer in an experimental animal in 1978 and a domestic animal in 1986. Notably, in 2002, we achieved a breakthrough with the first successful production and transfer of in vitro fertilization and sexing embryos ( Nguyen B X, 2006).

> Advancements in cryobiology, including the world's first groundbreaking achievement of successful rapid freezing of embryos after dehydration at room temperature in 1983 (Nguyen et al 1983, 1984; JP Renard et al, 1984) and the first successful vitrification of somatic cloning embryos in 2000 ( Nguyen et al, 2000)

> Significant contributions to stem cell biotechnology, achieving the first successful cryopreservation and differentiation of MSC cells during the period of 2008-2010.

> Implementing stem cell cryobanking technology and clinical application with hematology and blood transfusion hospitals, obstetrics hospitals and cancer hospitals - a premise for updating cutting-edge new C&G technologies.

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Signaling Pathways Impact on Induction of Corneal Epithelial-like Cells Derived from Human Wharton's Jelly Mesenchymal Stem Cells

Hong Thi Nguyen 1 2, Kasem Theerakittayakorn 1, Sirilak Somredngan 1,..., Ruttachuk Rungsiwiwut 8, Chuti Laowtammathron 9, Nguyen Xuan Bui 10, Rangsun Parnpai 1

Int J Mol Sci. 2022 Mar 12;23(6):3078.

The combination of RA, SB505124, BMP4, and EGF for the first 3 days of differentiation followed by supplementing hormonal epidermal medium for an additional 6 days could generate corneal epithelial-like cells that expressed a CEC specific marker CK12. This study reveals that WJ-MSCs have the potential to transdifferentiate into CECs which would be beneficial for further applications in LSCD treatment therapy.

Hair follicle aging is driven by transepidermal elimination of stem cells via COL17A1 proteolysis

Hiroyuki Matsumura, Yasuaki Mohri, Nguyen Thanh Binh, Hironobu Morinaga, Makoto Fukuda, Mayumi Ito, Sotaro Kurata, Jan Hoeijmakers, and Emi K. Nishimura

Quiescent and aging hair follicle stem cells

Stem cells enable normal cell homeostasis, but they also exist in a quiescent state, ready to proliferate and differentiate after tissue damage. Now, two studies reveal features of stem cells in the hair follicle, an epithelial mini-organ of the skin that is responsible for hair growth and recycling (see the Perspective by Chuong and Lei). Wang et al. found that the Foxc1 transcription factor is induced in activated hair follicle stem cells, which in turn promote Nfatc1 and BMP signaling, to reinforce quiescence. Matsumura et al. analyzed hair follicle stem cells during aging. They identified type XVII collagen (COL17A1) as key to hair thinning. DNA damage-induced depletion of COL17A1 triggered cell differentiation resulting in the shedding of epidermal keratinocytes from the skin surface. These changes then caused hair follicle shrinkage and hair loss.

Science, this issue p. 559, p. 613; see also p. 10.1126/science.aad4395

INTERNATIONAL SCIENTIFIC SEMINAR ON "DISEASES ASSOCIATED WITH AGENT ORANGE/DIOXIN EXPOSURE IN VIETNAM - CURRENT SITUATION AND SOLUTIONS TO IMPROVE EFFECTIVENESS OF PREVENTION AND TREATMENT"

Monday, 20 December 2021

Identification of biomarkers for dioxin exposure and dioxin-associated cancer susceptibility

Thi My Anh NEILDEZ-NGUYEN1,2, Xuan Nguyen BUI3

1 Centre de Recherche Saint-Antoine, INSERM UMRS 938, Sorbonne Université, Paris, France.

2 EPHE, PSL University, Paris, France.

3 South-East Asian Biotechnology Center, Hanoi, Vietnam.

Abstract

Dioxins are environmental pollutants that can accumulate in human tissue through the food chain. Exposure to 2,3,7,8-Tetrachlorodibenzo-para-dioxin (TCDD), the most toxic dioxin, as a contaminant in the defoliant Agent Orange, dumped during the Viet

Services and history

Research projects

• National projects R&D in Embryo Biotechnology (470107, 1980-1985; 52D-0113,1986-1990;KC-08-16); Vietnam National Foundation for Science and Technology Development (NAFOSTED) project on somatic cloning;

• project of cooperation with AIRE Development and INRA French on cryobiology and embryo preservation, embryo transfer; project for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), Japan for somatic cloning and vitrification cryopreservation; Vietnam_Italy Project on development of reproductive technology in buffalo; Vietnam-USA project on in vitro fertilization sexing and intercontionental embryo transfer.

• MOST Project ĐTĐL 2007/03 for differentiation of human umbilical cord matrix stem cell into neural and cardiac progenitor cells; Viet-Thailand project on induction of corneal epithelial cells;

• https://rep.bioscientifica.com/view/journals/rep/71/2/jrf_71_2_032.xml?rskey=b49amE&result=4; https://pubmed.ncbi.nlm.nih.gov/16725971/

• DOI: 10.1016/0093-691x(84)90459-x

https://pubmed.ncbi.nlm.nih.gov/10898213/

• https://www.publish.csiro.au/rd/RDv19n1Ab93

  https://pubmed.ncbi.nlm.nih.gov/25474756/

  doi.org/10.1111/asj.13650

  https://doi.org/10.3390/ijms23063078

  https://www.researchgate.net/publication/220023086

Transfer technology and Development project

• Project supported by AIRE-Developpement (International Agency for Research and Study for Development, French), INRA French, Bomed-USA for implementation of embryo biotechnology in domestic animal breeding.

• Project Biodiva Supported by FSP (Fonds de solidarité prioritaire), FFEM (Fonds français pour l'environnement mondial), CIRAT Centre de Coopération Internationale en Recherche Agronomique pour le Développement) and INRA for "Inventory, characterization and promotion of domestic animal biodiversity and wilderness of the Annamitic Cordillera in Vietnam, for conservation and sustainable development", improvement of somatic Cloning of wild Saola and Gaur, cryobank management and development of in situ - ex situ links.

• JSPS-Japan-VAST Vietnam Project for application vitro production, manipulation and preservation of porcine embryos of Vietnam's native pig breeds for the conservation of biodiversity in pigs.

• Project 01C-05/04-2016-3, supported by HanoiDOST. Main objective: Developing technique of reproductive management for improving rabbit production.

• https://pigtrop.cirad.fr/content/download/2581/13171/file;https://www.publish.csiro.au/RD/RDv16n1Ab259;https://www.publish.csiro.au/rd/ExportCitation/rdv17n2ab56; https://www.publish.csiro.au/RD/RDv16n1Ab259)

• https://123dok.net/document/qvl9g9ng-rapport-annuel-activit%C3%A9s-recherche-intensification-syst%C3%A8mes-elevage-vietnam.html

• https://webcache.googleusercontent.com/search?q=cache:NnWWfh_95q8J:https://agritrop.cirad.fr/549699/1/document_549699.pdf+&cd=1&hl=fr&ct=clnk&gl=vn&lr=lang_vi%7Clang_fr

• https://123dok.net/article/la-production-scientifique-et bibliographique.qvl9g9ng;

• https://www.semanticscholar.org/paper/The-scientific-production-of-the-Biodiva-project-to-Cuong-Maillard/71b3c971d14572ae168e58c1303d87f800361a10;

• https://agris.fao.org/agris-search/search.do?recordID=FR2013104863

• https://www.publish.csiro.au/rd/RDv18n2Ab77

• https://www.publish.csiro.au/rd/RDv19n1Ab308

• https://pigtrop.cirad.fr/content/download/2581/13171/file

https://dost.hanoi.gov.vn/tinhoatdongcuaso//news/0a1dDnsUqQBB/4/640482.html;jsessionid=sNcBt2-9cIyzVEEUYHzgzFL+.undefined

• https://pigtrop.cirad.fr/content/download/2581/13171/file

• DOI: 10.1530/jrf.0.0710573

• PMID: 16725971 DOI: 10.1016/0093-691x(84)90459-x

• N Bui-Xuan-Nguyen 1, Y Heyman, J P Renard. Direct freezing of cattle embryos after partial dehydration at room temperature Theriogenology. 1984 Oct;22(4):389-99. PMID: 16725971 DOI: 10.1016/0093-691x(84)90459-x

A new and simple method for freezing of bovine morulae and blastocysts was developed. Embryos were predehydrated at room temperature, frozen at -30 degrees C (cooling rate = 12 degrees C/min), and plunged into liquid nitrogen. This method was compared in vitro and in vivo to the slow freezing method (0.3 degrees C/min to -30 degrees C).

• Uoc, N. T. , Ty, L. V. , Tuoc, D. , Due, N. H. , Hanh, N. V. , Thanh, N. T. , Bui, L. C. , Laloy, E. , and Renard, J. P. (2002). Effect of tissue sampling conditions on the in vitro multiplication and reprogramming potential of somatic cells obtained from different specimens of the saola (Pseudoryx nghetinhensis) species. Theriogenology 57, 437.

• Hiroyuki Matsumura, Yasuaki Mohri, Nguyen Thanh Binh, Hironobu Morinaga, Makoto Fukuda, Mayumi Ito, Sotaro Kurata, Jan Hoeijmakers, Emi K Nishimura. Hair follicle aging is driven by transepidermal elimination of stem cells via COL17A1 proteolysis. Science. 2016 Feb 5;351(6273) doi: 10.1126/science.aad4395. PMID: 26912707 DOI: 10.1126/science.aad4395

• Hong Thi Nguyen , Kasem Theerakittayakorn, Sirilak Somredngan, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Pishyaporn Sritangos, Mariena Ketudat-Cairns, Sumeth Imsoonthornruksa ,Juthaporn Assawachananont , Nattawut Keeratibharat, Rangsirat Wongsan Ruttachuk Rungsiwiwut,Chuti Laowtammathron, Nguyen Xuan Bui and Rangsun Parnpai *. Signaling Pathways Impact on Induction of Corneal Epithelial-like Cells Derived from Human Wharton’s Jelly Mesenchymal Stem Cells. International Journal of Molecular Sciences, Int. J. Mol. Sci. 2022, 23, ,. https://doi.org/ 10.3390/ijms23063078

Nov 7, 2024

HOSTEP cGMP Platform Groundbreaking Ceremony

"This is a significant milestone in the strategy to develop stem cell technology in Vietnam, contributing to the establishment of a modern medical foundation based on advanced technology and dedicated to improving public health.

July 17, 2024

Signing of cooperation documents between HSC and Goliver, French

July 15, 2024

cGMP cleanroom design of HOSTEP platform

June 19, 2023

Approval of HOSTEP by Hoa Lac Hi-tech Park Management Board

According to Decision No. 78/QĐ-CNCHL dated June 19, 2023, the project has been approved and allocated to Lot No. RD6-3, located in the Research and Development (R&D) zone within Hoa Lac High-Tech Park, Hanoi, Vietnam. The project is slated to span a duration of 50 years and is eligible for favorable incentives related to high-tech initiatives.

2018-2023

HOSTEP proposal development

28/2/2018

Southeast Asia Biotechnology center/Hoa Lac Stem cell group and Biotalentum Ltd, Hungary signed a Memorandum of Understanding (MOU) on study on using stem cell based models to screen anti-tumor active substances from Vietnamese medicinal plants

Both parties agree to conduct and promote research activities to (1) establish stem-cell based models for drug screening including using the human glioblastoma cells for anticancer screening and using human induced pluripotent stem (iPSC)-derived Neuronal Stem cells (iPSC-NPC) and neurons for neurotoxicity screening, (2) apply the developed model for screening chemical compounds extracted and purified from Vietnamese medicinal natural resources with anticancer potential, (3) study the methods of transformation of IPSCs into glioblastoma (stem) cells, and (4) develop scientific plans for further applications of stem cell based models in pharmaceutical industry including patent application, publication of research results, training and transfer of technology.

10/10/2017

A stem-cell research consortium comprising HSC, Lung Lo 51 JSC, the National Institute of Hematology and Blood Transfusion (NIHBT), Hanoi Ngan Nam JSC, Hanoi Medical University (HMU), and 108 Military Central Hospital has entered into an agreement for collaborative scientific, technological, and innovative development cooperation

HSC takes the leading role in initiating and designing a project focused on 3D stem cell culturing.

27/9/2017

Hoa Lac Stem Cell JSC signed MOU with Hucord on a joint cooperation for stem cell resesearch and development in Vietnam

On September 27, 2017, Bui Xuan Nguyen, the General Director of HSC, signed a Memorandum of Understanding (MOU) with Yoon Jung-Cheol, CEO of Hucord Corporation (http://eng.hucord.com/), at Hoa Lac Hi-Tech Park in Vietnam, marking a collaborative effort in cord blood storage and stem cell research.

11/2015

The 12th Annual International Conference of the Asian Reproductive Biotechnology Society

Asia region has become a key driver of global politics and economics, but still faces critical challenges to increase the application of science, technology, innovation and partnership

3/8/2011

With the aim at promoting the development and application of stem cell technology in Vietnam, Vice Minister of Science and Technology cum Chairman of Hoa Lac Hi-tech Park, Dr. Nguyen Van Lang; Vice Minister of Health cum Director of the Central Obstetrics Hospital, Prof. Nguyen Viet Tien along with representatives of HSC Company; Hi-tech Business Incubator; and Nano Biology Center signed a memorandum of understanding to build and develop stem cell technology in Hoa Lac Hi-tech Park on 3 August, 2011.